Fig. 5.

Comparison of non-constant time sensitivity-enhanced a HSQC and b TROSY of selective ¹³C-enriched nucleotides dissolved in 95% w/v d8-glycerol at 30°C for all 4 rNMPs derived from K10 bacterial culture. Base correlations are depicted. The ribose C2′ resonances that normally resonate between 73.7 and 74.7 ppm and Cyt and Ura C5 resonances at 96.67 ppm and 102.69 ppm respectively (in a decoupled HSQC) are folded in. Identical acquisition and processing parameters were used. The time domain matrices were processed without apodization functions. As expected the TROSY peaks are right and down shifted from the decoupled HSQC peaks. Two resonances that are either very weak or absent in the HSQC spectrum are boxed