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Inhibition of tubulin assembly by 4e, 13a, and 13e in vitro. All compounds were tested at a concentration of 10 µM. Effects of compounds on tubulin polymerization were assessed by monitoring the increase in light scattering, measured as optical density (O.D.), at 340 nm. Standards of 10 µM nocodazole (a tubulin assembly inhibitor) and 10 µM paclitaxel (a tubulin assembly promoter) were used for direct comparison.
