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. Author manuscript; available in PMC: 2011 Apr 20.
Published in final edited form as: Dev Cell. 2010 Apr 20;18(4):556–568. doi: 10.1016/j.devcel.2010.02.006

Figure 1. chinmo is autonomously regulated by JAK/STAT pathway activity.

Figure 1

(A) chinmo mRNA is upregulated in cells anterior to the furrow in a third instar GMR-upd eye disc. (B) chinmo mRNA is absent at this stage in a wildtype (WT) eye disc. (C) Activity of the JAK/STAT pathway, as assessed by the 10xSTAT-dGFP transcriptional reporter, is not detected in a mid-third instar WT eye disc (arrows). (D,E) chinmo mRNA is expressed in a second instar eye disc, like 10xSTAT-dGFP. (F–F”) Expression of chinmo-lacZ (red) is autonomously lost in Stat92E clones, which lack GFP (pink arrowheads). (G) chinmo mRNA (black) is autonomously increased in a hop flip-out clone (hop FO) (red outline). (H–H”) Chinmo protein (red) is increased in hop FO clones (arrowheads). The furrow is marked by a green arrowhead in A–C,F,G. Dorsal is up and anterior to the left. (I) A dorsal view of a WT adult Drosophila head. (J,K) Animals with Stat92E− M+ clones in the eye-antennal disc exhibit small eyes, excess head cuticle, and are frequently headless. (L,M) Animals carrying chinmo− M+ clones also exhibit these phenotypes. Arrow points to an adult with only a rudimentary head (K,M). (N) Ser-LacZ expression (magenta) in a WT eye-antennal disc. Eyegone (Eyg) is green. (O,P) Ser-lacZ is ectopically expressed in the antennal disc in mosaic Stat92E (O’, arrowheads) or chinmo (P’, arrowheads) clones. Clones lack GFP in O,P.