LRRK2 binds to MKK3. a HEK 293 cells were transfected with V5-LRRK2 (WT) ± Flag-MKK3. After 24 h, the lysates were collected and V5-LRRK2 immunoprecipitated. The immunoprecipitates were immunoblotted with either anti-V5 antibody (upper panel, to detect LRRK2) or anti-Flag (lower panel, to detect MKK3). b HEK 293 cells were transfected with Flag-MKK3 ± LRRK2 (WT). After 24 h, the lysates were collected and Flag-tagged MKK3 immunoprecipitated. The immunoprecipitates were immunoblotted with either anti-V5 antibody (upper panel, to detect LRRK2) or anti-Flag (lower panel, to detect MKK3). Arrows point to LRRK2 and MKK3. Representative data from three independent experiments.