Table 3.
Oligonucleotides used in this study.
| Name | Sequence |
|---|---|
| CaJEN1Fwd | GCATGCTTAGCACTGGCACTGACGCGTATTAGTAAATAGACTTTAATTTAG CTTTTACCC |
| CaJEN1Rev | ATCGAATGCTTAACTGATCACGTCGACTTTTTAGTATTTGATTGAATT GAATTGGTTATAAGA |
| CaJEN2Fwd | GCATGCTTAGCACTGGCACTGACGCGTGAGCACTAACAATTAGTTGTACAGTTCAAAACTCCG |
| CaJEN2Rev | ATCGAATGCTTAACTGATCACGTCGACCCGTCTCATATTTCTAACCGATTGTGCCAGTGGCTC |
| DRPS10 | GTGGTTGGAGCTTTGATG |
| DCaJEN2Rev | AGCCATGAGAGCCATCTC |
| CaJEN2A1 | GGTGATACATATGGTAGA |
| CaJEN2A2 | GTGATCCACATTGGATGG |
| CaJEN2-S1Fwd | GGATGAATTGAAACAATATTCCTGGCACGAAGTTCTTAATCCGTTTGAACCATTAGTGGA CCTCTTCGCTATTACGCCAG |
| CaJEN2-S2Rev | CGGCACCTCTGTTTTCAGGACCAATAAAAACAACAAACATCAAGTAAGCCAAAACAGCAC GCAGATTACCCTGTTATCCCTA |
| DCaURA3-1Rev | CTGCTCTCTCACTATAGGTC |
| DCaHIS1-1Rev | CGGTCTGGTAAATGATTGAC |
| DCaJEN2lcFwd | CCCAATACATCACATTAC |
| CaJEN2-DB1Fwd | CTAACCATAGAAATATTATGACTGCTGCTGATACTCATTCTATCACTAGTGCTGATG TTC TTTTCCCAGTCACGACGTT |
| CaJEN2-DB2Rev | CCACCAACACTTACTCTTTATGTTCAACTTCTGGTTTTTCCAAAGTCTTTTGAG TAATATTAC TGTGGAATTGTGAGCGGATA |
| CaJEN2-DB3Fwd | CATAATAGACACATTATTCGTCCACCAAAATTCACTTGGCCCGCTATTCGAAAATATGCC TTTTCCCAGTCACGACGTT |
| CaJEN2-DB4Rev | CTTCAAGATCAGAATCACCTCTGTCTTCCCTATCACTATCGTACGCACTGTATTCGTCATC TGTGGAATTGTGAGCGGATA |
| DminiURA3Rev | TAGAAGGACCACCTTTGATTGT |
| CaJEN2GFPFwd | GAAGAAGGTAATATTACTCAAAAGACTTTGGAAAAACCAGAA GTTGAACATAAAGAG GGTGGTGGTTCTAAAGGTGAAGAATTATTC |
| CaJEN2GFPRev | CACACACATACTATTTTAACAAATCATAAACCCATTTATTATCAAAATAAACTATACTTG TCTAGAAGGACCACCTTTGATTG |
| DJEN2GFP_Fwd | CTTGGTCAGTGGTGCCAA |
| GFP Rev | AACATCACCATCTAATTCAAC |
| JEN2_416For | GGGATCCAATATTATGACTGCTGCTGATACTCATTCTATC |
| JEN2_416Rev | GAAGCTTTTAGTGATGGTGATGGTGATGCTCTTTATGTTCAACTTCTGGTTTTTC |
| JEN2416ForREQ | AAAACACCAAGAACTTAGTTTCGACGGATTCTAGAACTAGTGGATCCAATATTATGACTGCTGCTGATACTCATTCTATC |
| JEN2416RevREQ | CATGACTCGAGGTCGACGGTATCGATAAGCTTTTAGTGATGGTGATGGTGATGCTCTTTATGTTCAACTTCTGGTTTTTC |
| URA3-dpl200fwd | TAAAACGACGGCCAGTGAAT |
| URA3-dpl200rev | ACCATGATTACGCCAAGCTC |
| RPS10 | ACTAATTCTTCTCTTCAG |
| Ins CaJEN1 | AAGTCTATTTACTAATACG |
| TDH promoter | ACAAGGCAATTGACCCACGCATGTATCTA |
| CYC Terminator | GAATGTAAGCGTGACATAACTAATTACATG |
DNA sequences complementary to the sequences of pLUL and pLHL (S1/S2), pDDB57 (DB1/DB2/DB3/DB4) and pGFP-URA3 (F1/R1) are shown in bold.