Fig. 3.

Phylograms of unique fHbp amino acid sequences in modular variable segments V_A (residues 8–73) and V_B (residues 79–93). Segments derived from fHbp in the variant group 1 are shown as black circles, the variant 2 group as red circles, and the variant 3 group as light-blue circles. Where multiple proteins possessed an identical sequence in a segment, the number of proteins is given. A.β.9 and B.α.3 refer to proteins with exceptional junctional points (see text). Bar, two amino acid changes per 100 residues. The histogram (Segment V_A) shows the mean number of protein sequences (y axis) and percentage amino acid identity (x axis) generated by comparing each of the 48 α A segments with the corresponding α- and β-type A segments of all 70 proteins. The corresponding histograms comparing each of the α segments with the corresponding α and β types, and each of the β segments with the corresponding α and β types, for all five modular variable segments, V_A to V_E, are shown in Supplementary Fig. S1.