Table 2.

Amino acid identity within and between sequence types by segment

Variable segment	Residue range*	α Sequence types			β Sequence types			Identity between α and β types (%)
		Signature residues†	Number‡	Identity range (%)	Signature residues†	Number‡	Identity range (%)
VA	8–73	QSV	48	89–100	DSI and KDN	21	87–100	69–83
VB	79–93	IRQ	51	86–100	VQK	17	100	60–66
VC	98–159	QDS	38	83–100	NNP	32	93–100	37–45
VD	162–180	AG or AS	58	89–100	PN	12	84–100	52–63
VE	186–253	SLGI	38	83–100	HLAL	31	92–100	48–57

*Amino acid numbering based on the mature fHbp from strain MC58. (petide ID no. 1)

†See Supplementary Table S4 for complete sequences of each variable segment.

‡Number of sequences in each sequence type for each variable segment (n=70 proteins). Data from four segments with exceptional crossover points were excluded from calculations of percentage identity [segments A.β.9, B.α.3 (n=2) and E.β.10; see Figs 4, 5 and 6 and text].