Fig. 7.
Chromatograms of various CYP and UGT reaction substrates before and after incubation with rat liver microsomes for 1 h. The detector used was MS/MS and the methods were described in details in the Method section. (a) MRM chromatograms of testosterone after 1 h incubation in a CYP reaction system lacking NADPH (negative control); (b) MRM chromatograms of testosterone after 1 h incubation in a CYP reaction system; (c) MRM chromatograms of matrine after 1 h incubation in a CYP reaction system lacking NADPH (negative control); (d) MRM chromatograms of matrine after 1 h incubation in a CYP reaction system; (e) MRM chromatograms of genistein after 1 h incubation in a UGT reaction system lacking UDP-GA (negative control); (f) MRM chromatograms of genistein after 1 h incubation in a UGT reaction system; (g) MRM chromatograms of matrine after 1 h incubation in a UGT reaction system lacking UDP-GA (negative control); (h) MRM chromatograms of matrine after 1 h incubation in a UGT reaction system.
