Table 2.
Gene name, gene product size, and forward and reverse primer sequences used in GeXP assays of antioxidant and oxidative stress related genes in rat liver
| Gene Name | Fragment Size | Left Sequence w/Universals | Right Sequence w/Universals |
|---|---|---|---|
| Ubb | 187 | AGGTGACACTATAGAATAGACACCATCGAGAACGTGAA | GTACGACTCACTATAGGGAGACAAGGTGCAGGGTTGACT |
| 18S a | 194 | AGGTGACACTATAGAATAGCTCCAGGACGGAGTTCATA | GTACGACTCACTATAGGGACAGCAGGTGGAGCTCTGATT |
| Nfkbib | 204 | AGGTGACACTATAGAATACCCGAGGATGAGGATGATAA | GTACGACTCACTATAGGGATCATCAGGAAGAGGTTTGGC |
| Akr1b1 | 210 | AGGTGACACTATAGAATACGCAGAAGTCTGAAGCTGTG | GTACGACTCACTATAGGGACTGGTACTGCCCTCCACATT |
| Grm5 | 213 | AGGTGACACTATAGAATAGCCAACTTTAATGAGGCCAA | GTACGACTCACTATAGGGATGATGTACACCTTCGGGACA |
| Apo E | 230 | AGGTGACACTATAGAATAGAAGATGAAGGCTCTGTGGG | GTACGACTCACTATAGGGACTCTGCAGCTCTTCCTGGAC |
| Stip1 | 236 | AGGTGACACTATAGAATAACTACAACAAATGCCGGGAG | GTACGACTCACTATAGGGATGGCACTTCTTGAGCACATC |
| Cox11 | 244 | AGGTGACACTATAGAATACTTCCTTCCCCCATCTGTTT | GTACGACTCACTATAGGGACTGTTCCTCACAATGGCTCA |
| (Mt1a) | 250 | AGGTGACACTATAGAATACACCAGATCTCGGAATGGAC | GTACGACTCACTATAGGGAACTGTTCGTCACTTCAGGCA |
| Oxsr1 | 257 | AGGTGACACTATAGAATACAGCGATTGAACTAGCCACA | GTACGACTCACTATAGGGATTGTGCCTCAACAGTTCTGC |
| GPX | 271 | AGGTGACACTATAGAATATCAACATCGAGCCTGACATC | GTACGACTCACTATAGGGACAGACTTAGAGCCCCCAGTG |
| Gapdh a | 279 | AGGTGACACTATAGAATAATCAATGGATTTGGACGCAT | GTACGACTCACTATAGGGAAGCTCCAGGGGATTTCCTTA |
| SOD1 | 285 | AGGTGACACTATAGAATACTTGCTTTTTGCTCTCCCAG | GTACGACTCACTATAGGGAAAAATGAGGTCCTGCAGTGG |
| Hao1 | 293 | AGGTGACACTATAGAATACCTGTCAGACCATGGGAACT | GTACGACTCACTATAGGGATGAGCTGTGGTGGTAGCTTG |
| NADH | 298 | AGGTGACACTATAGAATAGTGAAGCCCATTTTCAGTCG | GTACGACTCACTATAGGGATAATGTGTGTCCGCTGCTTC |
| Actb a | 307 | AGGTGACACTATAGAATAATGTACGTAGCCATCCAGGC | GTACGACTCACTATAGGGAAGGGCAACATAGCACAGCTT |
| Cat | 314 | AGGTGACACTATAGAATAGTGGTTTTCACCGACGAGAT | GTACGACTCACTATAGGGACACGAGGTCCCAGTTACCAT |
| Knar b | 325 | AGGTGACACTATAGAATAATCATCAGCATTGCATTCGATTCCTGTTTG | GTACGACTCACTATAGGGAATTCCGACTCGTCCAACATC |
a Gene used for normalization
b Internal control