Table 2.
Results of rapid test (ELISA TeSeE), immunoblot (TeSeE Western Blot), IHC (cocktail of 2G11 and F89/160.1.5) and PrP genotypes of AS cases.
| 2004 | 2005 | 2008 | 2009 | |||||
|---|---|---|---|---|---|---|---|---|
| M7 | M72 | M45 | M31 | M15 | M19-1 | M27 | M9-2 | |
| Origin | Fallen stock | Fallen stock | Slaughtered | Slaughtered | Fallen stock (Advanced autolysis) | Fallen stock | Slaughtered | Slaughtered |
| Month of death | June | November | April | January | February | December | February | April |
| Province | Vizcaya | Alava | Guipuzcoa | Guipuzcoa | Alava | Guipuzcoa | Guipuzcoa | Guipuzcoa |
| Age (years) | 6.3 | 12.8 | 4.3 | 7.0 | 9.1 | 5.6 | 8.1 | 10.3 |
| Genotype | AFRQ ALRQ |
ALRR ALRQ |
AFRQ ALRQ |
AFRQ AFRQ |
AFRQ AFRQ |
AFRQ ALHQ |
ALRQ ALRH |
ALRQ ALRQ |
| Clinical symptoms | Not registered | Not registered | Not registered | Yes | Not registered | Not registered | Not registered | Not registered |
|
Rapid test result (cut off) |
0.377 (0.218) |
0.387 (0.219) |
1.112 (0.219) |
2.788 (0.230) |
2.859 (0.230) |
2.710 (0.219) |
0.498 (0.102) |
1.289 (0.103) |
| WB TeSeE | ||||||||
| Obex-Cerebellum | 1 | 2 | 3 | 2 | 3 | 1 | 1 | 2 |
| Cerebellum | 0 | 0 | 3 | 3 | 1 | 3 | 1 | 3 |
| Cerebral Cortex | 0 | 0 | 1 | 0 | 2 | 1 | 1(3)* | 2 |
| IHC | ||||||||
| MO | PC | NA | NA | 2 | 1 | 1 | 1 | 1 |
| Cerebellum | 1 | 3 | 3 | 3 | 1 | 3 | 1 | 3 |
| Frontal Lobe | PC | NA | 2 | NA | 3 | 1 | 2 | 1 |
Numbers on WB column indicates the signal intensity according to Figure 1. 0: negative, 1: faint, 2: moderate and 3: intense. NA: Not available. PC: Poor condition. MO: Medulla oblongata. Immunohistochemical staining was graded subjectively for intensity and spread from 0 to 3 as in WB. * Refers to PrPSc signal after concentrating the sample 10 folds.