Table 1.

erbB antibodies used

Antibody	Source	Type	Clone	Reactant	Positive control	Dilution	Incubation time and temperature
NCL-EGFR-384	Novocastra, Newcastle-upon-Tyne, UK	Monoclonal	EGFR.25	EGFR (c-erbB1 protein) (internal domain)	Human skin	1:100	60 min. at room temperature
NCL-EGFR	Novocastra	Monoclonal	EGFR.113	EGFR (c-erbB1 protein) (external domain)	Human skin	1:10	60 min. at room temperature
c-erbB-2	Novocastra	Monoclonal	CB11	c-erbB2 protein (internal domain)	SKBR-3 breast cancer cell line	1:40	60 min. at room temperature
c-erbB-2	Immunotech, Marseille, France	Monoclonal	3B5	c-erbB2 protein (internal domain)	SKBR-3 breast cancer cell line	Ready to use	60 min. at room temperature
c-erbB-2	Novocastra	Monoclonal	5A2	c-erbB2 protein (internal domain)	SKBR-3 breast cancer cell line	1:50	60 min. at room temperature
NCL-c-erbB-3	Novocastra	Monoclonal	RTJ1	c-erbB-3 protein	Normal kidney	1:10	Over night at 4°C
c-erbB-4/HER4	NeoMarkers (Fremont, CA, USA)	Monoclonal	HFR-1	c-erbB-4 protein	Breast carcinoma	1:10	Over night at 4°C