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. Author manuscript; available in PMC: 2010 Apr 26.
Published in final edited form as: Int J Neuropsychopharmacol. 2009 May 13;12(10):1395–1408. doi: 10.1017/S146114570900042X

Fig. 1.

Fig. 1

Relative mRNA expression of NMDAR subunits in adult rat PFC and parvalbumin-immunoreactive (PV-ir) interneurons. (a) mRNA expression of NMDAR subunits in the PFC tissue. NR2B mRNA expression (31.7±3.91, n=8) is significantly higher than that of the NR2A subunit (1.92±0.66, n=8, p=0.0001). (b, c) High-magnification photographs showing the PV-ir interneurons (arrows) labelled with rapid NovaRED immunostaining before (b) and after (c) the laser cut. Scale bar in (c)=50 μm. (d, e) RNA integrity numbers (RIN) were measured with Agilent Bioanalyzer and the electropherogram of RNA extracted from laser microdissection (LMD)-picked PV-ir interneurons is shown in (d), where 5S covers the small rRNA fragments (5S and 5.8S rRNA and tRNA) and 18S and 28S cover the 18S peak and 28S peak (e). RIN=8.63±0.16 (n=9) with 28S/18S=1.90±0.15 and 18S/baseline=7.23±1.17. (f) RT–PCR amplification of PV (150 bp) showing the expression of PV in LMD-captured PV-ir cells compared to that in PV-negative tissue. The left lane was the molecular weight marker with 100-bp intervals. (g) Relative mRNA expression of NMDAR subunits in PV-ir interneurons. The mRNA level of NR2A subunit was significantly higher than that of NR2B subunit (p=0.031). # Indicates that NR2D mRNA was at an undetectable level (* p<0.05, ** p<0.01).