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. Author manuscript; available in PMC: 2010 Apr 26.
Published in final edited form as: J Biol Chem. 2007 Jul 3;282(35):25950–25959. doi: 10.1074/jbc.M703311200

FIGURE 5. EPR spectra of CopZ.

FIGURE 5

A chemically reduced CopZ, pH 7.0; B, chemically reduced CopZ, pH 10; C, C77S variant; D, C75S variant; E, C109S variant; F, C119S variant; G, after 77 K cryoreduction (CR) of diferric CopZ. Sharp feature at ~ 1500 G in some spectra is non-heme Fe(III). The sharp feature at ~ 1500 G in some spectra is non-heme Fe(III). Conditions are as follows: T=10 K; frequency, 9.372 GHz; power, 1 milliwatt; modulation amplitude, 5 G. WT, wild type.