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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Invest Radiol. 2009 Aug;44(8):433–439. doi: 10.1097/RLI.0b013e3181a4d7e9

Figure 4. Effects of U0126 and LY294002 on proliferation, MMP-1 production, and TIMP-1 production in control and Omniscan-treated fibroblasts.

Figure 4

Cells were incubated under control conditions or in the presence of Omniscan with or without U0126 or LY294002 for three days. At the end of the incubation period, culture fluid was collected and assayed as follows. Upper panel. Cell proliferation assessed as cell counts. Middle panel. MMP-1 assessed by western blotting. Inset: Western blot from one experiment. Lower panel. TIMP-1 assessed by ELISA. Values shown are means and standard errors based on five separate experiments. Statistical significance of the data was assessed by ANOVA, followed by paired-group comparisons. *indicates statistically significant increase compared to control at p<0.05 level. **indicates statistically significant increase compared to Omniscan alone at p<0.05 level.