Table 2.

Label-free quantitative MS protein identification results

Protein priority	Peptide ID confidence	Multiple sequences	Median number of sequences	Number of proteins
1	HIGH	YES	7	56
2	HIGH	NO	1	149
3	MODERATE	YES	2	11
4	MODERATE	NO	1	218
Overall			1	434

Proteins identified by LC-MS/MS were assigned to one of four categories based on the confidence in their identity, with priority 1 the highest confidence and priority 4 the lowest. To be categorized as priority 1, peptide ID confidence must be high (>90%) and multiple unique peptide sequences (median number per group listed) must be determined. Priority 4 requires a single peptide with ID confidence (>70%). Altogether, 434 proteins were identified. (Statistical summary provided by INCAPS, K. Bemis).