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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: New Phytol. 2010 Apr;186(1):207–215. doi: 10.1111/j.1469-8137.2010.03187.x

Fig. 3.

Fig. 3

Decreased levels of miRNA accumulation in dcl1(Allo) and ago1(Allo) RNAi lines. (a) Representative small RNA blots of selected miRNAs (miR156a, miR159a, miR159b, miR171a, and miR403) and tasiRNA (tasiR255) in dcl1(Allo):RNAi and ago1(Allo):RNAi lines. Averaged numbers (from two independent blots) indicate relative densitometric intensities of miRNA levels between the RNAi lines and a control transgenic plant. The hybridization signals were normalized using the U6 as an endogenous control. Densitometric intensities were obtained using imagequant software (Bio-Rad). (b) Averaged relative expression levels (REL) (mean ± SE) of miRNAs and tasiRNA shown in (a). The REL of each miRNA or tasiRNA was averaged from densitometric intensities in four dcl1(Allo):RNAi lines or ago1(Allo):RNAi lines, which was normalized to the transgenic line containing empty vector construct (dashed line).