FIGURE 4.

Effects of isoproterenol (Iso) and forskolin (Fsk) on the phosphorylation of RAF in carbachol- and PMA-treated rat parotid acinar cells. A and B, cells were exposed to agents as in Fig. 3. Cell lysates were used to immunoprecipitate c-RAF-1, which was subjected to immunoblot analysis using two different phospho-RAF antibodies and c-RAF-1, as indicated. C and D, quantification of RAF phosphorylation on Ser-338 (C) and Ser-259 (D) were calculated relative to the basal control (no additions). For C, n = 6–7. **, p < 0.01 versus basal; *p < 0.01 versus carbachol control; #, p < 0.03 versus PMA control. For D, n = 5. *, p < 0.03 versus basal control; #, p < 0.01 versus basal control.