FIGURE 3.

THAP1 and THAP3 belong to a large family of HCF-1-binding THAP-zf proteins. A, multiple alignment of the HBMs found in human THAP-zf proteins. A consensus is shown. B, primary structure of the nine human THAP-zf proteins containing consensus HBMs or HBM-like motifs. The asterisk indicates the position of the HBM, which is always located 4–9 amino acids upstream the predicted coiled-coil domain. C, two-hybrid assay. A yeast strain expressing the GAL4DB-HCF-1 kelch domain was transformed with constructs expressing the indicated human THAP-zf proteins or control protein CREB3. Transformants were patched onto SD-Leu/Trp (LT) and SD-Leu/Trp/His (LTH) plates. D, β-galactosidase assays. The β-galactosidase activity of each strain is expressed as relative light units per second (RLU/s). The result shown for each strain is the average of two independent colonies after subtraction of the background level obtained from the GAL4DB-HCF-1 kelch strain transformed with pGADT7 vector alone.