FIGURE 7.

Pyk2 activation is necessary for full induction of cell motility. A, HCLE cells were transfected with control or Pyk2 siRNA or were infected with control or PRNK-expressing adenovirus prior to wound healing assays in the presence of 25 units/ml apyrase and 10 ng/ml EGF (EGF) as indicated. The percentage change in healing from corresponding untreated or EGF-treated control was plotted. *, p < 0.001 (significant difference from untreated controls); **, p < 0.05 (significant difference from EGF-treated controls); ND, no significant difference from EGF-treated controls (p > 0.05). Columns are means, and error bars are S.D. B, immunofluorescence microscopy of cells treated as in A. Images were acquired with a ×20 objective. All cells stained for F-actin, but only PRNK-expressing cells stained for FLAG.