TABLE 1.
S. cerevisiae strains used in this study
| Name | Genotype | Reference |
|---|---|---|
| SM1058 | Mata trp1 leu2 ura3 his4 can1 | Ref. 10 |
| SM1188 | Ste14-4::TRP1, isogenic to SM1058 | Ref. 10 |
| CH2704a | 2μ URA3 PPGK-His10-myc3N-STE14 | Ref. 30 |
| CH2714a | 2μ URA3 PPGK-His10-myc3N | Ref. 30 |
| CH2866a | 2μ LEU2 PPGK-STE14 | This study |
| CH2865a | 2μ URA3 PPGK-His10-myc3N-STE14 + 2μ LEU2 PPGK-STE14 | This study |
| CH2870a | 2μ URA3 PPGK-His10-myc3N-STE14-L81F | This study |
| CH2867a | 2μ URA3 PPGK-His10-myc3N-STE14-L81F + 2μ LEU2 PPGK-STE14 | This study |
| CH2781a | 2μ URA3 PPGK-His10-myc3N-STE14-E213Q | This study |
| CH2899a | 2μ URA3 PPGK-His10-myc3N-STE14-E213Q + 2μ LEU2 PPGK-STE14 | This study |
| SM3495a | 2μ URA3 STE14 | Ref. 13 |
a Strains are isogenic to SM1188. PGK-phosphoglycerate kinase promoter, His10-10 histidine residues, myc3-three myc epitopes, N-tags located on the amino terminus.