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. 2010 Mar 1;285(18):13397–13404. doi: 10.1074/jbc.M109.083246

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — TAB2 scaffolds TAK1 and NLK to promote functions of NLK. A, TAB2, but not TAB2-ΔM, cooperates with TAK1 in activating kinase activity of NLK. B, overexpression of TAB2, but not TAB2-ΔM (ΔM), leads to an increase in phosphorylation of LEF1. WT, wild type. FLAG-tagged NLK or LEF1 was co-transfected with other plasmids and immunoprecipitated for in vitro kinase assay (IVK) of NLK autophosphorylation (A) or LEF1 phosphorylation (B). The results of in vitro kinase assay were visualized with phosphorimaging. C and D, shown are the effects of TAB2, TAB2-ΔM, and TAB2-C3 on the TAK1-NLK cascade-mediated LEF1 polyubiquitylation (Ub). To check the polyubiquitylated LEF1, the indicated plasmids were co-transfected into HEK293T cells, and LEF1-FLAG was immunoprecipitated with FLAG antibody and resolved to Western blot. Polyubiquitylated LEF1 (immunoblotted against HA) was shown at 100–250 kDa.