FIGURE 1.

Topology of NBCe1-A and selective sequence alignment of NBCe1-A residues mutated in pRTA among the SLC4 bicarbonate transporters. A, topology of NBCe1-A based on in vitro glycosylation analysis. The branched structure indicates the two N-linked glycosylation sites. The positions of 8 residues of mutated pRTA are depicted as filled circles. B, amino acid sequence of NBCe1-A and SLC4 bicarbonate transporters were aligned with the Clusterclaw program. Residues mutated in NBCe1-A causing pRTA are highlighted in bold. Note that SLC4A11 is not included in the alignment because it does not transport bicarbonate.