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A2M protects SP-D(n/CRD) from cleavage by elastase. A, intact A2M fully protects 5 μg of SP-D(n/CRD) from elastase (0.5 units) cleavage up to 32.25 μg/ml within 2 h. B, methylamine cleaved A2M also protects SP-D(n/CRD) from cleavage by elastase. SP-D(n/CRD) (5 μg) was incubated with elastase (0.05 units) in the presence of either methylamine cleaved or intact A2M (100 μg/ml). Both the intact and the cleaved A2M protect SP-D(n/CRD) from degradation by elastase.
