FIGURE 4.
Differential potency of NBD-containing peptides. Purified recombinant GST-NEMO-(2–200) (2 nm) was incubated with purified recombinant C terminus FLAG-tagged full-length IKK-2 (2 nm). The ability of WT or mutant (W739A and W741A) NBD peptides to disrupt NEMO-IKK-2 binding was measured by Alphascreen. The data represent the mean ±S.E. of four independent experiments.