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. 2010 Feb 18;285(18):13498–13506. doi: 10.1074/jbc.M109.099895

FIGURE 4.

FIGURE 4.

Differential potency of NBD-containing peptides. Purified recombinant GST-NEMO-(2–200) (2 nm) was incubated with purified recombinant C terminus FLAG-tagged full-length IKK-2 (2 nm). The ability of WT or mutant (W739A and W741A) NBD peptides to disrupt NEMO-IKK-2 binding was measured by Alphascreen. The data represent the mean ±S.E. of four independent experiments.