FIGURE 5.

PPARγ is critical for PEDF-dependent NFκB activation. PPARγ antagonist interferes with IκB-α phosphorylation in the presence of PEDF. HMVECs were pretreated 30 min with proteasome inhibitor MG132 (10 μm) and then treated 25 min with the indicated combinations of bFGF (10 ng/ml), PEDF (10 nm), and T0070907 (1 μm). A, whole cell extracts were subjected to Western blot analysis for IκB-α. B, HMVECs plated on coverslips were treated as in A and stained for p65/RelA. NFκB nuclear localization is indicated with white arrows. C, cells were activated with VEGF (0.5 ng/ml) and treated with PEDF or the 34-mer (10 nm). NFκB p65/RelA was measured in the whole cell extracts by Western blot.