FIGURE 4.

CEH overexpression decreases the proinflammatory cytokine production from macrophages. A, thioglycollate-elicited macrophages were obtained from Ldlr^−/− and Ldlr^−/−CEHTg mice and lipid loaded with acetylated low density lipoprotein (AcLDL; 50 μg/ml). IL-1β and MCP-1 levels in the conditioned medium were determined by ELISA. Data are expressed as mean ± S.D. (n = 3). B, thioglycollate-elicited macrophages were obtained from Western diet-fed Ldlr^−/− and Ldlr^−/−CEHTg mice, and total RNA was isolated from adherent cells 2 h after plating. Expression of IL-1β, IL-6, and MCP-1 was determined by real time reverse transcription-PCR, and data (mean ± S.D.; n = 3) are expressed as percent mRNA levels in Ldlr^−/−CEHTg mice. *, p < 0.05.