FIGURE 8.

Transgenic overexpression of CEH reduces macrophage infiltration into adipose tissue. Infiltration of macrophages into perigonadal adipose tissue was assessed by immunohistochemistry as well as by measuring macrophage-specific gene expression as described under “Experimental Procedures.” A, macrophages stained with F4/80 (red) surround the adipocytes. Nuclei are stained with DAPI (blue). The magnification for all the three images is the same (scale bar shown in one of the images), although the field size is different. B, quantification of crown-like structures (CLS). Data are expressed as mean ± S.D. for n = 3; a minimum of three fields were analyzed for each animal. C, total RNA from adipose tissue was used to determine the levels of macrophage-specific genes (CD-68 and SR-A) by real time reverse transcription-PCR. Data (mean ± S.D., n = 3) are presented as percent Ldlr^−/− control. *, p < 0.05.