FIGURE 5.

A simplified representation of an AGP glycomodule (A), and the effect of arabinose (Ara) removal on the ability of these glycomodules to act as acceptors in FUT assay (B). A, the simplified structure of arabinogalactan polymers in BY2 AGPs was adapted from Ref. 32. The Araf residues susceptible to mild acid treatment are indicated by asterisks. B, fucosyltransferase assays were performed using as acceptors mild untreated and acid-treated AGPs-BY2WT (100 μg) in the presence of GDP-[¹⁴C]Fuc and detergent extracts from BY2 WT cells or transgenic BY2 cell lines expressing the AtFUT4.1 or AtFUT6 genes. The reactions containing no protein extracts were used as negative controls. Each value is an average based on at least three experimental sets. The error bars represent the S.E.