TABLE 2.
Substrate specificity of AtFUT4.1 and AtFUT6 enzymes
Each acceptor (100 μg) was tested for [14C]Fuc incorporation in the presence of extracts from transgenic cell lines expressing AtFUT4.1 or AtFUT6 genes. Fucosyltransferase assays were conducted as described in Ref. 17. Extracts from BY2 WT cells were used as a control. The values are the averages of at least three experiments from two biological replicates. The values are the amounts of [14C]Fuc incorporated (pmol/h/mg of protein). The standard deviations are indicated.
| Acceptor added (100 μg) | Source of enzyme extract |
||
|---|---|---|---|
| BY2 WT | BY2 expressing AtFUT6 | BY2 expressing AtFUT4.1 | |
| None | 2 ± 1 | 6 ± 1 | 7.5 ± 2 |
| AGPs-BY2WT | 12 ± 3 | 485 ± 10 | 210 ± 5 |
| RGI from potato | 80 ± 9 | 100 ± 5 | 87 ± 6 |
| RG from soybean | 109 ± 5 | 94 ± 8 | 95 ± 2 |
| Arabinan | 67 ± 9 | 65 ± 6 | 66 ± 5 |
| Tamarind XyG | 49 ± 9 | 39 ± 5 | 37 ± 2 |
| N-Acetyllactosamine | 122 ± 10 | 111 ± 9 | 107 ± 5 |
| α1-acid glycoprotein | 4 ± 1 | 3.5 ± 1 | 3 ± 1 |