FIGURE 1.
Effect of forskolin on glutathionylation of the Na+-K+ pump β1 subunit and α1/β1 subunit interaction. A, the immunoblot shows biotinylated GSS-β1 subunit after exposure of myocytes loaded with biotin-GSH to forskolin for 5, 15, or 30 min or vehicle control. B, shown is the effect of forskolin on glutathionylation of the β1 subunit as shown by immunoblotting (IB) the β1 subunit immunoprecipitate (IP) with GSH antibody. The immunoblot of the cell lysate and non-immune IgG control are also shown. C, control. C, shown is the effect of SOD on forskolin-induced glutathionylation as detected by the biotin-GSH technique. D, shown is the effect of forskolin on Na+-K+ pump α1/β1 subunit co-immunoprecipitation. Representative α1 and β1 subunit immunoblots after immunoprecipitation with β1 subunit antibody are shown. The histograms show mean densitometry of blots from three-four experiments, each normalized against control (%). IP indicates the antibody used for immunoprecipitation. IB indicates the antibody used for immunoblot. The asterisk indicates significant difference versus control.