FIGURE 5.

Effect of forskolin on co-immunoprecipitation of ϵPKC with RACK. Myocytes exposed to Ang II (positive control), forskolin, or vehicle control were lysed before co-immunoprecipitation. ϵPKC immunoprecipitate (IP) was immunoblotted (IB) for ϵPKC or RACK. The histogram shows the mean densitometric measurements of immunoblots from three experiments standardized to the value of control samples. Ang II induced an increase in ϵPKC/RACK co-immunoprecipitation that was insensitive to H-89. Forskolin also induced an increase in ϵPKC/RACK co-immunoprecipitation, but this was abolished by H-89. The asterisk indicates significant differences compared with control. C, control.