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Western blot analysis of total and phosphorylated GSK-3β, β-catenin, GATA-4, NFATc3, and NFATc4 in WT and TEAD-1 Tg hearts. Total protein extracts (100 μg) obtained from fetal, neonatal, and 5- and 10-month-old WT and TEAD-1 Tg (line 12) hearts revealed a significant decrease in phospho-GSK-3β at all ages and no change in total GSK-3β (A). TEAD-1 Tg neonatal (day 14) hearts displayed a significant decrease in nuclear β-catenin, GATA-4, NFATc3- and NFATc4 (B) (n = 4).
