TABLE 5.
Expression profile of wild type and transgenic hearts
Microarray analysis of TEAD-1 Tg (line 12, n = 3) versus WT 10-month-old hearts showed significant changes in the mRNA expression of collagen, extracellular matrix, and calcium handling genes.
| Cardiac remodeling | |||
|---|---|---|---|
| Gene title | WT | Fold | p value |
| Calcium handling | |||
| Calcium channel, voltage-dependent, α 2/δ subunit 3 | 1 | 3.0 | 0.037 |
| Phospholamban | 1 | 0.7 | 0.003 |
| Ryanodine receptor 2, cardiac (Ryr2), mRNA | 1 | 2.1 | 0.000 |
| RyR1 mRNA for skeletal muscle ryanodine receptor | 1 | 12.1 | 0.030 |
| Sarco/endoplasmic reticulum Ca2+-ATPase, Serca2a | 1 | 0.7 | 0.020 |
| Sarco/endoplasmic reticulum Ca2+-ATPase; Serca2b | 1 | 0.5 | 0.000 |
| Triadin | 1 | 0.5 | 0.022 |
| Troponin C, cardiac/slow skeletal | 1 | 0.7 | 0.014 |
| Collagen and extracellular matrix | |||
| A disintegrin and metallopeptidase domain 23 | 1 | 0.5 | 0.009 |
| Annexin A2 | 1 | 1.7 | 0.020 |
| Biglycan | 1 | 5.7 | 0.002 |
| Coagulation factor II (thrombin) receptor | 1 | 2.0 | 0.022 |
| Collagen, type I, α 1 | 1 | 2.4 | 0.004 |
| Collagen, type I, α 2 | 1 | 3.3 | 0.000 |
| Collagen, type III, α 1 | 1 | 3.3 | 0.007 |
| Collagen, type V, α 2 | 1 | 3.0 | 0.005 |
| Collagen, type VIII, α 1 | 1 | 4.3 | 0.016 |
| Collagen, type VIII, α 2 | 1 | 3.1 | 0.002 |
| Collagen, type XIV, α 1 | 1 | 1.7 | 0.037 |
| Connective tissue growth factor | 1 | 4.1 | 0.048 |
| Fibrillin 1 | 1 | 3.2 | 0.003 |
| Fibronectin type III domain containing 3B | 1 | 2.1 | 0.035 |
| Hydroxysteroid (17-β ) dehydrogenase 12 | 1 | 1.7 | 0.031 |
| Integrin β 1 (fibronectin receptor β) | 1 | 2.8 | 0.007 |
| Integrin β 1-binding protein 1 | 1 | 1.5 | 0.007 |
| Matrix metallopeptidase 2 | 1 | 2.1 | 0.006 |
| Microtubule-associated serine/threonine kinase 2 | 1 | 0.5 | 0.029 |
| Microtubule-associated protein 1 light chain 3α | 1 | 0.6 | 0.006 |
| Microtubule-associated protein 1B | 1 | 2.6 | 0.039 |
| Microtubule-associated protein 4 | 1 | 1.7 | 0.026 |
| Microtubule-associated protein, RP/EB family, member 1 | 1 | 1.8 | 0.003 |
| Procollagen C-endopeptidase enhancer protein | 1 | 2.6 | 0.019 |
| Procollagen-proline, 2-oxoglutarate 4-dioxygenase, α 1 | 1 | 2.1 | 0.013 |
| Procollagen-proline, 2-oxoglutarate 4-dioxygenase, α II | 1 | 1.6 | 0.030 |
| TGFβ 2 | 1 | 4.0 | 0.017 |
| TGFβ 3 | 1 | 2.7 | 0.012 |
| Thrombospondin 1 | 1 | 4.3 | 0.003 |
| Thrombospondin 2 | 1 | 0.4 | 0.011 |
| Thrombospondin 4 | 1 | 4.7 | 0.025 |
| Tissue inhibitor of metalloproteinase 1 (TIMP1) | 1 | 2.5 | 0.002 |
| Tissue inhibitor of metalloproteinase 2 (TIMP2) | 1 | 2.4 | 0.002 |