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. 2010 Mar 5;285(18):13781–13787. doi: 10.1074/jbc.M110.107300

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Expression of Ca²⁺-handling proteins in Wt and RyR-null primary myotubes. Left, representative Western blots using antibodies directed against RyR1, PMCA, NCX3, SERCA1, myosin (to demonstrate similar differentiation state), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a loading control. Right, expression of RyR1 associated with a significant decrease in the expression of SERCA and NCX3 and a significant up-regulation of the expression of PMCA. Data are expressed as mean ± S.D., n = 5 Western blots/group.