FIGURE 1.

Coprecipitation of CNX and PDI with Env using anti-Env Abs. Lysate of BHK-21 cells (the corresponding cell number is indicated) expressing Env was submitted (no pulldown) to SDS-PAGE analysis and WB using anti-Env (A), -CNX (B), or -PDI (C) Abs. Alternatively, lysate was incubated with irrelevant sheep IgG (anti-Env⁻) or D7324 Ab (anti-Env⁺) prior to precipitation, elution using loading buffer, SDS-PAGE, and WB as indicated. A, *, nonspecific species detected by the peroxidase-labeled anti-sheep Ab staining system.