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. 2010 Mar 5;285(18):13839–13849. doi: 10.1074/jbc.M109.097345

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Oligomerization of VAP-B enhances the production of VAP-B(P56S)-insoluble aggregates. A, the indicated Myc-tagged VAP-B proteins were expressed in HeLa cells and their distribution between the Triton X-100-soluble (S) and insoluble (P) fractions was assessed by Western blotting using anti-Myc antibody. Densitometric analysis (software: ImageJ) of the Western blot signals was used to estimate the relative distribution of each mutant between the soluble and insoluble fractions (B). C, the P56S/ΔCC/II mutant did not form visible aggregates. The indicated mutants were expressed in HeLa cells, fixed in 4% paraformaldehyde, immunostained with anti-Myc antibody, and analyzed by confocal microscope. Bar, 10 μm.