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Exogenous FGF1 treatment could rescue the decrease of neurosphere formation. A, representative images of neurospheres with different treatments are shown. Scale bar, 100 μm. B, neurospheres generated by U-1240 MG/F1BGFP(+) after pSG5 empty vector and pHA-RFX1 transfection. FGF1 (20 ng/ml or 100 ng/ml) were treated in the neurosphere medium. n = 3; *, p < 0.05.
