Skip to main content
. Author manuscript; available in PMC: 2010 Apr 26.
Published in final edited form as: Oncogene. 2004 Mar 11;23(10):1863–1873. doi: 10.1038/sj.onc.1207309

Figure 6.

Figure 6

Kinetics of carcinoma (SCC) formation in transgenic and double transgenic mice.

Groups of K5-CDK4 transgenic, K5-CDK4/K5-Cyclin D1double transgenic and wild type sibling mice were initiated with DMBA and promoted with multiple applications of TPA on dorsal mouse skin. (A), Percentage of mice with carcinomas as a function of weeks of study. (+) K5-Cyclin D1, (◆) K5-Cyclin D1/K5-CDK4 doubles transgenic, (▲) K5-CDK4 and (■) Wild type mice. (B), Western blot analysis of CDK4 and cyclin D1 expression in papillomas from K5-CDK4/K5-cyclin D1 double transgenic mice (K4/D1), K5-CDK4 single transgenic (K4), K5-cyclin D1 (D1) single transgenic and wild type mice (WT). Protein lysates of papillomas separated by SDS-PAGE and blotted to a nitrocellulose membrane. Primary antibodies against CDK4 and cyclin D1 were used in the immunoblotting analysis.