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. Author manuscript; available in PMC: 2011 Apr 15.
Published in final edited form as: Anal Chem. 2010 Apr 15;82(8):3183–3190. doi: 10.1021/ac902683t

Figure 3.

Figure 3

Characterization of droplet generation and PCR amplification using MEGAs. (A) 32-channel MEGA chip. Left: photo of the cross-shaped nozzles each generating ~4 nL droplets at 5.6 Hz (6.4 × 105 droplets per hour). Middle: size distribution of uniform droplets collected from eight nozzles in a device. RSD: relative standard deviation. Right: a representative flow cytometric analysis of 624 bp amplicons on beads amplified from pUC18 template (1 copy/droplet) in ~3 nL droplets generated from four nozzles in a device. (B) 96-channel MEGA. Left: image of droplet production at the T-shaped nozzles with a total throughput of 2.4 × 106 droplets per hour. Middle: size distribution of uniform ~2 nL droplets collected from sixteen nozzles in a device. Right: a representative flow cytometric histogram of beads carrying the FAM labeled PCR product from E. coli K12 at 0.2 cells per droplet (cpd) in 2.5 nL droplets. For all tests of droplet generation, the mock PCR mix containing ~100 beads per uL was used.