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EGFR amplification pre-exists in a subset of PC9GR4 cells. High-throughput FISH analysis of PC9GR4 cells demonstrates that the majority of cells contain no EGFR amplification (A) while a subpopulation (6%) harbor clustered (arrow) EGFR amplification (B) similar in appearance to the PC9DR2 cells (see Figure S4B). EGFR (CTD-2257H21; green); MET (RP-11-95I120; orange); CEP 7 (aqua).
