Figure 2.

Effects of rapamycin on nucleophosmin expression. A, rapamycin (Rap; 100 nmol/L) was added as indicated to asynchronous WT MEFs. Forty-eight hours after rapamycin treatment, cells were harvested and proteins were separated by SDS-PAGE and immunoblotted with antibodies specific for nucleophosmin, γ-tubulin, and phospho-S6 ( ). B, WT MEFs were infected with retroviruses encoding β-galactosidase (EV) and Ras^V12. Rapamycin (100 nmol/L) was added as indicated 24 h postinfection. All samples were collected 48 h postinfection and proteins were immunoblotted with antibodies against γ-tubulin, Ras, and nucleophosmin.