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In situ hybridization. Panels A-C shows results from Sense probe negative controls with no detectable nuclear staining. Panels D-F the positive cells of tissue sections probed with HPV-L1, EBV-EBER, and KSHV-ORF72 showed dark brown staining of the nucleus. The probes did not detect all viruses in all tumor cells, however, HPV, EBV, and KSHV were detected in some regions. Panels G-I shows a higher magnification of 60× compared to panels D-F which showed magnification of 40×.
