Figure 5.
qPCR of infection with 16S rRNA gene variant 2 of A. phagocytophilum. Cyclic bacteraemia of persistent A. phagocytophilum infection in five lambs (L6-L10) inoculated with the Norwegian 16S rRNA gene variant (GenBank no) AF336220 monitored by qPCR for six months. The horizontal line shows the threshold for bacteraemia and represents the lowest (10 copies) plasmid dilution for the standard curve calibration. The results are presented as logarithm transformed means of triplicate Cq readings (X) for each sample, calculated as Log10 (1+X).