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. 2010 Apr 15;52(1):25. doi: 10.1186/1751-0147-52-25

Table 1.

Primer -and plasmid sequences for qPCR of the A. phagocytophilum msp2 (p44) expression site (partial sequence).

Primer Sequence Conc. Tm °C
ApMSP2252 5'ACAGTCCAGCGTTTAGCAAGA-3' 0.5 μM 57.0
ApMSP2459 5'GCACCACCAATACCATAACCA-3' 0.5 μM 56.5
Plasmid construct
5'GAATTCGCCCTTACAGTCCAGCGTTTAGCAAGATAAGAGATTTTAGTA
TAAGGGAGAGTAACGGAGAGACTAAGGCAGTATATCCATACTTAAAGGA
TGGAAAGAGTGTAAAGCTTGAGTCTAACAAGTTTGACTGGAACACTCCTG
ATCCTCGGATTGGGTTTAAGGACAACATGCTTGTAGCTATGGAAGGCAGT
GTTGGTTATGGTATTGGTGGTGCCAGGGTTGAGCTTGAGATTGGTTACG
AGCGCTTCAAGACCAAAAGGGCGAATTCT-3'

Primer binding sites are indicated (underscored) in the sequence of the plasmid construct. The concentrations of primers are calculated for the final reaction mix.