Summary diagram and phylogenetic postitions of the genomic markers interrogated against the Ghana MTC strain collection. Shown are the various major divisions of the MTC segregated according to the presence or absence of inter-species-, intra-species-, and sublineage-specific polymorphisms. Circles are placed at points in evolutionary history beyond which each strain that was evaluated possessed a consistent set of polymorphisms. The nodes are numbered in the figure as follows to denote: 1. RD12can, 3' cfp32 deletion; 2. TbD1, narGHJI -215; 3. pks15/1 (7-bp deletion), katG463; 4. undefined deletion at the RDRio/MiD3 locus; 5. gyrA95; 6. RD9, gyrB1450; 7. RD713, TbD1197, aroA285, Rv1332523; 8. RD711; 9. RD7, RD8, RD10, pks15/1 (6-bp deletion), katG203, 3'cfp32311, RD13174, PPE552148, PPE552154; 10. Rv15101129; 11. RD701, RD702, hsp65540, nat751; 12. rpoB1163; 13. rpoB1049; 14. RD1das; 15. mmpL6551; 16. MiD3; 17. RD12, RD13; 18. RD4; 19. RD1BCG. Lineages that include strains from the Ghana collection are terminated with arrowheads. Note that distances are arbitrary and do not reflect the number of phylogenetically relevant polymorphisms present at each juncture. TbD1-positive M. tuberculosis is also known as "ancient" M. tuberculosis and TbD1-negative M. tuberculosis is also known as "modern" M. tuberculosis [1]. WA - West African.