Table 1.
Micro-Tom tissues used for RNA preparation
Organ | Treatment | Tissue | Condition | Library |
---|---|---|---|---|
Leaf | CMV(strain TN) and satellite RNA | Inoculated leaves, systemic leaves | 16 dpi (21-day old) | LEFL1 |
ToMV (strain Lta1, L11A, L2a, Ltb1, LJB) | Inoculated leaves, systemic leaves | 7, 14 dpi (28, 35-day old) | ||
Non-pathogenic Alternaria alternata | Inoculated leaves | 6, 24 hpi (5-week old) | ||
Alternaria alternate f. sp. lycopersici (As-27) | Inoculated leaves | 6, 24 hpi (5-week old) | ||
Corynespora cassiicola (isolated from tomato and cucumber) | Inoculated leaves | 6, 24 hpi (3-week old) | ||
Cladosporium fulvum (strain 210, 211, 217) | Inoculated leaves | 5 dpi (40-day old) | ||
Fusarium oxysporum f. sp. lycopersici race 1 and 2 | Leaves | Inoculated to root, 7 dpi (4-week old) | ||
Non-pathogenic Fusarium oxysporum | Leaves | Inoculated to root, 7 dpi (4-week old) | ||
Pseudomonas syringae pv. tomato (DC3000 wild type), pv tabaci isolate 6605 (wild type, ΔfliC, ΔfliD) | Spray-inoculated leaves | 24 hpi (6-week old) | ||
Flagellin from P. syringae pv. tabaci isolate 6605, fgl22 | Sprayed leaves | 3 hps (6-week old) | ||
Probenazole (100 μg/ml) | Sprayed leaves | 4 dps (4-week old) | ||
Validamycin A (100 μg/ml) | Sprayed leaves | 4 dps (4-week old) | ||
Acibenzolar-S-methyl (100 μg/ml) | Sprayed leaves | 4 dps (4-week old) | ||
Validoxylamine A (100 μg/ml) | Sprayed leaves | 4 dps (4-week old) | ||
Salicilic acid (0.5 mM) | Sprayed leaves | 2 dps (4-week old) | ||
Methyl jasmonic acid (50 μM) | Sprayed leaves | 2 dps (4-week old) | ||
Fruit | Mature green | Pericarp | 40 daa, year 2003, 2004 | FC/LEFL2 |
Breaker | Pericarp | 42 daa, year 2003, 2004 | ||
Turning | Pericarp | 48 daa, year 2003, 2004 | ||
Red ripe | Pericarp | 50 daa, year 2003, 2004 | ||
Root | Roots from plants without flower | Roots | 6, 10-week old | LEFL3 |
Roots from flowered plants | Roots | 10, 12- week old | ||
Fusarium oxysporum race 2 | Roots | 1, 7, 14, 21 dpi, 4- to 11-week old |
Abbreviations: dpi, days post inoculation; hpi, hours post inoculation; hps, hour post spray; dps, days post spray; daa, days after anthesis. More detailed dexcription of the samples is available as Additional file 1.