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. Author manuscript; available in PMC: 2010 Apr 26.
Published in final edited form as: Int Immunol. 1996 Jul;8(7):1121–1129. doi: 10.1093/intimm/8.7.1121

Fig. 2.

Fig. 2

CD22 is restricted to the lgM+ B cell fraction in the bone marrow. Bone marrow cells were spun through Fico-Lite-LM and stained with FITC–B220, Texas Red–anti-IgM and biotin–anti-CD22 plus PE–avidin. The histograms in the right side of the figure illustrate the expression of CD22 on the pro- and pre- (A), immature (B), and mature (C) B cell populations The gates for each of these populations are shown in the left hand panel The plots are derived from the small light scatter gated cells The isotype control for CD22 staining is shown in (C). Mean normalized fluorescence intensity values demonstrated that populations A and B expressed 8.3 ± 1 5% (SD) and 12.5 ± 2 8% CD22 levels respectively when compared with population C [(100%) average raw scale value of 5 1 ± 0 9] One of eight experiments