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. 2010 Mar 10;30(10):3589–3599. doi: 10.1523/JNEUROSCI.3732-09.2010

Figure 4.

Figure 4.

Inhibition of HDAC activity alters histone H3 trimethylation after contextual fear conditioning. A, Experimental design is outlined with results displayed in B to D. B, HDAC inhibition with NaB treatment 1 h before fear conditioning (NaB + CS) increased freezing levels compared with vehicle-treated cohorts (Student's two-tailed t test, t(12) = 2.524, p = 0.0267, n = 7/group). *p value significant compared with vehicle-treated context plus shock alone. C, Histone H3 trimethylation (H3K4me3) levels slightly increased with NaB treatment when compared with vehicle-treated naive animal cohorts (one-way ANOVA with Tukey's multiple-comparison test, F(2,11) = 13.64, p = 0.0019, n = 4/group). **p < 0.01 compared with vehicle-treated naive controls, ns = not significant compared with (context plus shock; CS). D, Dimethylated histone H3 (H3K9me2) levels significantly decreased with NaB treatment with fear conditioning (NaB + CS) compared with vehicle-treated fear-conditioned animals (context plus shock; CS) or vehicle-treated naive animal cohorts (one-way ANOVA with Tukey's multiple-comparison test, F(2,11) = 7.199, p = 0.0136, n = 4/group). *p < 0.05 compared with naive controls, #p < 0.05 compared with context plus shock. Solid line represents normalized naive control levels. Error bars are SEM.