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Time-dependent formation of methyladenosine (●), methionine (▲), 5′-deoxyadenosine (■), and S-adenosylhomocysteine (♦) in the RlmN-catalyzed methylation. (A) Formation of methyladenosine and methionine was monitored in the reaction of RlmN with [3H-methyl]-SAM. (B) Formation of 5′-deoxyadenosine and S-adenosylhomocysteine was monitored in the reaction of RlmN with [2,8-3H-adenosyl]-SAM.
