Fig. 5.

Supplementary. Reconstruction results of several well-defined micro-objects are illustrated to better quantify the resolution of the presented lensfree incoherent holographic imaging platform. The cross-sectional profiles along the dashed lines are shown on the images. For test feature 1, the FWHM values of the linear gap between the squares are 1.43μm and 0.6μm for the amplitude reconstruction and 40X objective-lens (NA = 0.6) microscope images, respectively. The same gap cannot be resolved by the 10X objective-lens (NA = 0.2). This indicates that the ~0.6 μm wide gap has been imaged with a spatial resolution of <1.5 μm using our incoherent lensfree holography approach. For test feature 2, the FWHM is measured on the cross-section across the letter “L”, and the values for amplitude reconstruction and 40X microscope images are 2.6 μm and 2.0 μm, respectively. The FWHM of the spacing between squares in test feature 3 is 1.80 μm and 1.95μm for amplitude reconstruction and 40X images, respectively. For all test features, we utilized z₁ = ~3 cm, z₂ = ~0.6 mm, F = ~1, D = 50 µm and spatially incoherent source at λ₀=470 nm with a FWHM spectral bandwidth of ~5nm. Exposure time in these experiments was ~25 ms. Scale bars are 5 µm for the reconstructed images as well as their microscope comparisons, and scale bars are 50 μm for the raw holograms on the left column.